"Protein CoAlation: a novel post-translational modification in redox regulation"
The main focus of my research is on the regulation of cell growth and metabolism via the mTOR/S6K pathway and CoA biosynthesis/homeostasis in health and disease. We have recently discovered a novel function of Coenzyme A in redox regulation mediated by covalent attachment of CoA to cellular proteins, which we termed protein CoAlation. We found that protein CoAlation is a reversible post-translational modification which is induced in mammalian cells by oxidizing agents and metabolic stress. To demonstrate that protein CoAlation is a common post-translational modification, we developed a strategy for the identification of CoA-modified proteins in cells and tissues. This approach allowed us to identify more than 500 CoAlated proteins in eukaryotic and prokaryotic cells. Significant number of CoAlated proteins are metabolic enzymes and proteins implicated in protein synthesis and anti-oxidative stress response. We also showed that a number of proteins identified by MS-MS are CoAlated in vitro and in vivo in ways that modified their activities. These findings suggest that under physiological conditions CoA functions as a major metabolic integrator via the production of metabolically-active derivatives, but can also act as a cellular antioxidant in response to oxidative or metabolic stress. Protein CoAlation has the potential to emerge as a novel field of research in redox regulation and signaling. We are currently investigating the mechanisms of protein CoAlation/deCoAlation and the role of CoA in balancing oxidative stress, redox state and cellular metabolism in health and disease.
Date:
11 July 2017, 13:00
Venue:
John Radcliffe Women's Centre, Headington OX3 9DU
Venue Details:
Anne Anderson Lecture Theatre, Level 3
Speaker:
Professor Ivan Gout (University College London)
Organising department:
Nuffield Department of Women's and Reproductive Health
Organiser:
Dr. Karl Morten (University of Oxford )
Organiser contact email address:
susie.barber@obs-gyn.ox.ac.uk
Topics:
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Susie Barber
