The chemical glycosylation of adenovirus DNA vaccine vectors - targeting DCs and protecting from host immunity
Viruses are powerful vectors for the delivery of nucleic acids, with applications in gene therapy and vaccination. However, major challenges for this technology include mis-targeting of the vector and neutralization by host antibody responses. Here we show that chemical addition of synthetic glycans to adenovirus (Ad) increased resistance to neutralizing antibody, and reset viral tropism to target macrophages and dendritic cells (DCs) with high selectivity for the complementary sugar receptors, thereby enhancing gene delivery. In vaccination studies, DC targeted glyco-virus enhanced antigen specific T cell responses. Since manipulation of this chemical glycosylation process is facile, it provides a flexible and potentially universal solution to key obstacles facing the utilization of viral vectors in therapeutic and vaccination contexts.
Date:
10 November 2015, 13:00 (Tuesday, 5th week, Michaelmas 2015)
Venue:
Sir William Dunn School of Pathology, South Parks Road OX1 3RE
Venue Details:
EPA seminar room
Speaker:
Leanne Minall (University of Oxford)
Organising department:
Sir William Dunn School of Pathology
Organisers:
Rebecca Moore (University of Oxford),
Helen Farr (University of Oxford),
Joanna Miller (University of Oxford),
Eva Gluenz (University of Oxford),
Kenny Moore (University of Oxford),
Rachel Exley (University of Oxford)
Part of:
Bug Sessions in infectious disease
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Rebecca Moore
