In this presentation, I’ll share data generated on the transport of enterocin DD14 (EntDD14), which doesn’t seem to take place, via classical pathways, namely ABC transport systems. In addition, I will discuss the impact of polynucleotide phosphorylase (PNPase), a major 3’-5’ exoribonuclease, on EntDD14 production and on the global transcriptome of the producing bacterium, Enterococcus faecalis 14. Finally, I’ll briefly mention possible antiviral and antibacterial applications of this bacteriocin.