Developing approaches to explore proteome complexity in the human brain
This seminar will be held on Microsoft Teams. Please join with your video off and mikes muted. Email hod-pa@dpag.ox.ac.uk for more details.
The explosion of new functional genomic technologies has revolutionised our approach to systems biology and highlighted a number of novel targets in neurodegenerative and, to a lesser extent, psychiatric diseases. However, the exact structure of a protein, its sub-cellular location, and post-translation modifications are critical to defining its function. Traditionally these properties have been defined through focused characterization of a single protein or protein complex. Mass-spectrometry proteomics enables high-throughput profiling of the larger proteome, but is often applied to bulk tissue, with quantification summarised to the level of protein groups. Here I will argue that this level of analysis inadequately captures the complexity of protein abundance in the human brain, and will talk about my experience developing methods to improve the resolution of proteomic techniques. I will describe analytical approaches to quantification of protein isoforms via the integration of high-throughput transcriptomics and proteomics, and the use of biochemical methods for the sub-cellular localisation of proteins in post-mortem human tissue.
Date:
11 May 2021, 15:00 (Tuesday, 3rd week, Trinity 2021)
Venue:
This seminar will be held on Microsoft Teams. Please email hod-pa@dpag.ox.ac.uk for more details and the link to join.
Speaker:
Dr Becky Carlyle (Massachusetts General Hospital, Harvard University)
Organising department:
Department of Physiology, Anatomy and Genetics (DPAG)
Organiser:
Professor Richard Wade-Martins (Professor of Molecular Neuroscience, University of Oxford)
Organiser contact email address:
hod-pa@dpag.ox.ac.uk
Hosts:
Professor David Paterson (DPAG, University of Oxford),
Professor Richard Wade-Martins (Professor of Molecular Neuroscience, University of Oxford)
Part of:
Neuroscience Theme Guest Speakers (DPAG)
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Talitha Smith
