Epi-microRNA mediated metabolic remodelling ensures affinity maturation
No virtual option for this seminar
Germinal center (GC)-B cell proliferation relies on oxidative phosphorylation. Positively selected GC-B cells initiate cell division in the hypoxic light zone (LZ) microenvironment and continue vigorous proliferation upon migration to the dark zone. However, the mechanisms underlying how these GC-B cells reprogram mitochondrial bioenergetic functions to sustain cell division while overcoming hypoxia-driven energy stress are not understood. We found that microRNA (miR)-155 directly repressed the expression of hypoxia-induced histone lysine demethylase 2a, resulting in fine-tuning of histone H3 di-methylated lysine 36 levels. This optimized the expression of vital nuclear mitochondrial genes in LZ GC-B cells, thereby preventing excessive mitochondrial reactive oxygen species production and apoptosis. Thus, miR-155-regulated epigenetic mechanisms functions as a metabolic switch facilitating dynamic mitochondrial remodelling of LZ GC-B cells, ensuring positive selection and affinity maturation.
Date: 13 November 2023, 12:00 (Monday, 6th week, Michaelmas 2023)
Venue: Kennedy Institute of Rheumatology, Headington OX3 7FY
Venue Details: Lecture Theatre
Speaker: Dr Dinis Calado (Senior Group Leader, The Francis Crick Institute (London, UK))
Organising department: Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences (NDORMS)
Organiser: Doris Chan (Kennedy Institute of Rheumatology)
Organiser contact email address: doris.chan@kennedy.ox.ac.uk
Host: Alexander Clarke (Kennedy Institute, University of Oxford)
Part of: Kennedy Institute Seminars
Booking required?: Not required
Audience: Members of the University only
Editor: Doris Chan