Detection of RNA in the central and peripheral nervous system using the RNAscope® in situ hybridization assay Application of RNAscope® and BaseScope™ in Neuroscience Research
The nervous system consists of numerous specialized cell types that remain to fully cataloged and
characterized at the molecular level. Due to the high degree of structural and functional
heterogeneity and the intricate spatial organization of these cells, it is of special importance to
analyze gene expression in the presence of full morphological and spatial contexts. Due to the lack
of specific antibody reagents, especially for lncRNAs, G-protein coupled receptors (GPCRs), and ion
channels, mapping of specific transcripts by in situ hybridization offers an excellent alternative
approach. The RNAscope® assay provides a powerful method to detect gene expression within the
spatial and morphological tissue context. BaseScopeTM is a novel in situ hybridization technology
that allows visualization of splice junctions between adjacent exons and/or retained introns in highly
specific and sensitive manner, allowing characterization of alternative splicing and circular RNAs in
cells and tissues.
Date: 24 May 2017, 12:00 (Wednesday, 5th week, Trinity 2017)
Venue: John Radcliffe West Wing and Children's Hospital, Headington OX3 9DU
Venue Details: Seminar Room A/B, Level 6, West Wing, John Radcliffe Hospital
Speakers: Faraia Shah (ACD Bio), Matthew Nolan (University of Oxford)
Organising department: Nuffield Department of Clinical Neurosciences
Organiser: Matthew Nolan (University of Oxford)
Organiser contact email address: fshah@acdbio.com
Host: Matthew Nolan (University of Oxford)
Booking required?: Not required
Audience: Members of the University only
Editor: Matthew Nolan