Unravelling virus replication-induced organelle remodelling by cellular cryo-electron tomography
Viruses developed various strategies to remodel infected cells to carry out genome replication and generate new progeny. While positive single-stranded RNA viruses hijack membranous organelles to form replication vesicles, many negative single-stranded RNA viruses use liquid organelles as a site of viral genome replication. We apply cellular cryo-electron tomography and correlative light and electron microscopy to study the replication and assembly of SARS-CoV-2 and Ebola viruses. We show that SARS-CoV-2 nsp3-4 are minimal components of the pore spanning double-membrane vesicles. In addition, we revealed Ebola virus nucleocapsid assembly inside membrane-less inclusion bodies using subtomogram averaging
Date: 24 November 2023, 14:00 (Friday, 7th week, Michaelmas 2023)
Venue: Medical Sciences Teaching Centre, off South Parks Road OX1 3PL
Venue Details: Lecture Theatre
Speaker: Dr Petr Chlanda (Schaller Research Group Department of Infectious Diseases - Virology Heidelberg University Hospital)
Organising department: Sir William Dunn School of Pathology
Organiser: Melissa Wright (Sir William Dunn School of Pathology)
Organiser contact email address: melissa.wright@path.ox.ac.uk
Host: Prof Ervin Fodor (Sir William Dunn School of Pathology)
Part of: Dunn School of Pathology Departmental Seminars
Booking required?: Not required
Audience: Members of the University only
Editor: Melissa Wright