Seminar by Dr Tanmay Bharat 'Structural studies of bacterial biofilm formation'
This seminar will be held virtually via Zoom. This seminar is open only to members of Oxford University, register with a valid Oxford University email address at https://medsci.zoom.us/meeting/register/tJEvduygrTgjEtEgg016bIiNc2-2j8TWstcg
Most bacteria on this planet are found within macroscopic, surface-attached, multi-cellular communities known as biofilms. Biofilms represent a key developmental state of prokaryotic existence, and understanding the fundamental molecular and cell biology of how biofilms are formed is an outstanding problem, relevant to understanding several important natural processes including human infections and the maintenance of microbiomes.

My laboratory uses structural biology techniques, coupled with high-resolution imaging to study general principles governing bacterial biofilm formation. We use electron cryomicroscopy (cryo-EM) and tomography (cryo-ET) to resolve structures of molecules that mediate biofilm formation. Correlated light and electron microscopy (CLEM) and mass spectrometry are used to support our investigations. We combine in vitro reconstitution of key molecules with in situ imaging to understand how pathogenic bacteria such as Pseudomonas aeruginosa form biofilms.

One of the central techniques used in the laboratory is cryo-ET, and we are heavily involved in developing methods for in situ macromolecular structure determination using cryo-ET. We have developed several imaging and image-processing techniques, which we have used to solve structures of macromolecules in situ directly in their cellular context. Method development efforts in the laboratory are driven by, and are highly complementary to the biological focus on understanding biofilm formation.

In this seminar, I will provide an update of recent results in the laboratory in studying bacterial biofilms.
Date: 14 February 2022, 12:00 (Monday, 5th week, Hilary 2022)
Venue: This seminar will be held virtually via Zoom. This seminar is open only to members of Oxford University, register with a valid Oxford University email address at https://medsci.zoom.us/meeting/register/tJEvduygrTgjEtEgg016bIiNc2-2j8TWstcg
Speaker: Dr Tanmay Bharat (Sir William Dunn School of Pathology, Oxford University)
Organising department: Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences (NDORMS)
Organisers: Ellen Mobbs (Kennedy Institute of Rheumatology), Dr Jelena Bezbradica (The Kennedy Institute of Rheumatology), Professor Irina Udalova (Kennedy Institute of Rheumatology)
Organiser contact email address: ellen.mobbs@kennedy.ox.ac.uk
Host: Professor Fiona Powrie (Kennedy Institute of Rheumatlogy)
Part of: Kennedy Institute Seminars
Booking required?: Required
Booking url: https://medsci.zoom.us/meeting/register/tJEvduygrTgjEtEgg016bIiNc2-2j8TWstcg
Audience: Members of the University only
Editors: Elizabeth Thompson, Ellen Mobbs