Seminar by Prof Joerg Enderlein 'Metal and Graphene Induced Energy Transfer Imaging for 3D Super-Resolution Microscopy'
This is a virtual seminar via Zoom, open only to members of Oxford University. Register using a valid Oxford University email address at https://medsci.zoom.us/meeting/register/tJcvcuCrpz0tHN1_ILK6oc73HoiEidCf6khN
Metal-Induced Energy Transfer (MIET) Imaging is a recently developed method that allows for nanometre resolution along the optical axis. It is based on the fact that, when placing a fluorescent molecule close to a metal, its fluorescence properties change dramatically, due to electromagnetic coupling of its excited state to surface plasmons in the metal. This is very similar to Förster Resonance Energy Transfer (FRET) where the fluorescence properties of a donor are changed by the proximity of an acceptor that can resonantly absorb energy emitted by the donor. In particular, one observes a strongly modified lifetime of its excited state. This coupling between an excited emitter and a metal film is strongly dependent on the emitter’s distance from the metal. We have used this effect for mapping the basal membrane of live cells with an axial accuracy of ~3 nm. The method is easy to implement and does not require any change to a conventional fluorescence lifetime microscope; it can be applied to any biological system of interest, and is compatible with most other super-resolution microscopy techniques that enhance the lateral resolution of imaging. Moreover, it is even applicable to localizing individual molecules, thus offering the prospect of three-dimensional single-molecule localization microscopy with nanometre isotropic resolution for structural biology. I will also present latest developments of MIET where we use a single layer of graphene instead of a metal film that allows for increasing the spatial resolution down to few Ångströms (Graphene-Induced Energy Transfer or GIET). In combination with single-molecule localization microscopy methods such as dSTORM or PAINT, MIET/GIET imaging offers nanometric isotropic resolution for bioimaging of molecular complexes and cellular structures.
Date: 24 January 2022, 12:00
Venue: This seminar is open only to members of Oxford University, register using a valid Oxford University email address https://medsci.zoom.us/meeting/register/tJcvcuCrpz0tHN1_ILK6oc73HoiEidCf6khN
Speaker: Prof Joerg Enderlein (Gottingen University)
Organising department: Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences (NDORMS)
Organisers: Ellen Mobbs (Kennedy Institute of Rheumatology), Jelena Bezbradica Mirkovic (KTRR SENIOR RESEARCH FELLOW), Professor Irina Udalova (Kennedy Institute of Rheumatology)
Organiser contact email address: ellen.mobbs@kennedy.ox.ac.uk
Host: Dr Marco Fritzsche (University of Oxford)
Part of: Kennedy Institute Seminars
Booking required?: Required
Booking url: https://medsci.zoom.us/meeting/register/tJcvcuCrpz0tHN1_ILK6oc73HoiEidCf6khN
Audience: Members of the University only
Editors: Elizabeth Thompson, Ellen Mobbs