Genetic interaction mapping of HIV host factors
Quantitative mapping of genetic interactions (GIs), by simultaneously perturbing gene pairs then measuring the resultant phenotypes, is a powerful tool for understanding complex biological phenomena permitting the unbiased characterization of gene function and the mapping of functional modules and pathways. This discussion will present a GI analysis pipeline to study the functions of HIV host factors through pairwise knockdown of 360 host genes (representing 129,600 combinations) followed by infection with HIV. This work combines a luminescence-based viral infectivity assay to quantify HIV infection with a microscopy-based cellular analysis, allowing us to assemble GI maps of the host factor complexes and pathways mediating the early HIV lifecycle.
Date:
1 July 2015, 15:00 (Wednesday, 10th week, Trinity 2015)
Venue:
Sir William Dunn School of Pathology, South Parks Road OX1 3RE
Venue Details:
EPA seminar room
Speaker:
Dr David Gordon (UCSF)
Organising department:
Sir William Dunn School of Pathology
Organiser contact email address:
edward.hutchinson@path.ox.ac.uk
Part of:
Bug Sessions in infectious disease
Booking required?:
Not required
Audience:
Members of the University only
Editor:
Edward Hutchinson
